Establishment and application of prevention and control methods against parasites of fish cultured in the Philippines
2005
The efficacy of bath treatment methods against the ectoparasites Amyloodinium ocellatum, Pseudorhabdosynochus lantauensis and Zeylanicobdella arugamensis on marine fish was determined under laboratory conditions. The toxicity of formalin and hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) to orange-spotted grouper (Epinephelus coioides) were also investigated. Freshwater or 200 ppm H<sub>2</sub>O<sub>2</sub> bath treatments for 1 hr were effective against the dinoflagellate A. ocellatum on hatchery-reared mangrove red snapper (Lutjanus argentimaculatus). Hydrogen peroxide at 200 ppm for 1 hr was effective in eliminating the gill monogenan P. lantauensis on E. coioides fingerlings. In vitro treatments of the marine leech Z. arugamensis using 250 ppm formalin for 3 hr significantly killed the parasite. Chlorine at 50 ppm for 1 hr can be used to disinfect leech-contaminated facilities. The computed 3 hr and 6 hr LC<sub>10</sub> (10% lethal concentration) of formalin to E. coioides fingerlings is 322 ppm and 275 ppm, respectively, at 28°C. The 9 hr LC<sub>10</sub> and LC<sub>50</sub> (median lethal concentration) of H<sub>2</sub>O<sub>2</sub> to grouper are 255 and 298 ppm at 28°C.
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