Immunohistochemical studies of epidermal differentiation, cell proliferation and p53 mutation in bovine ocular squamous cell carcinoma
2002
Vala, Helena | Afonso, A. F. | Esteves, F. | Santos, C. | Seixas, C. | Amaral, M. | Albuquerque, T. | Botelho, A. | Amado, A.
The purpose of the present study was to immunocharacterize Bovine Ocular Squamous Cell Carcinoma neoplastic cells, in terms of proliferation index and DNA damage, using two human primary antibodies anti- Ki67 antigen of the cell cycle and anti- p53. Ki67 is a nuclear antigen expressed in dividing cells during late G1, G2, S-phase and M-phase that disapears rapidly after mitosis. p53 is a tumor supressor protein that plays a key role in the control of the DNA damage acting as a supressor of tumour cell proliferation, enhancing genetic stability. Mutation of the gene that codes for p53 provides an extended intracellular half-life of the protein and greater stability, therefore increasing its expression. Tumours for this study were obtained at the slautherhouse, in Ponta Delgada, Azores. A total of thirty samples were colected from eighteen bovine. Immunohistochemical analysis was performed in parafin embeded sections of formalin fixed material, using the Avidin-Biotin-Peroxidase method (ABC). Primary antibodies used were anti-human p53 (NCL – p53 CM-1, NOVOCASTRA, UK) and anti-human Ki67 (NCL – Ki67p, NOVOCASTRA, UK), both policlonal. Positivity for both Ki67 and p53 was evaluated quantitatively and semi-quantitatively, respectively.
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