Immunohistochemical detection of filaggrin in the skin of different species
2007
Bardagí, M. | Vala, Helena | Fondevila, D. | Ferrer, L.
Filaggrin (FLG) is a key protein that facilitates terminal differentiation of the epidermis and formation of the skin barrier. Recent researches have demonstrated that, in humans, some variants of FLG are a major predisposing factor for atopic dermatitis. Furthermore, in immunohistochemical studies, FLG’s staining intensity was found significantly reduced in non-lesional and lesional atopic skin: FLG did not reach the horny layers and stained primarily granules in the stratum corneum. To the knowledge of the authors, the location of FLG in the skin of the domestic animals has not been investigated. Therefore, the aim of this research was to investigate, using immunohistochemistry, the expression of FLG in the skin of the dog, cat, horse, cow, pig and chicken. The long term purpose of this research line would be to investigate FLG expression in canine atopic skin. Formalin fixed, paraffin-emebedded sections were used in a ABC immunohistochemical technique. A monoclonal antibody against human FLG (Acrist Antibodies, Germany) was used as primary antibody. The results were very consistent in all species. FLG was detected clearly in the granular layer of the epidermis and in the outer root sheath of the hair follicles. In the chicken, all suprabasal layers were positive for FLG. The staining in all cases was cytoplasmic, being also the keratohyalin granules strongly stained. These results are very similar top those described in human beings and open the door to further researches regarding the FLG expression in animal skin diseases
显示更多 [+] 显示较少 [-]