Characterization of the Novel Phage vB_VpaP_FE11 and Its Potential Role in Controlling <i>Vibrio parahaemolyticus</i> Biofilms
2022
Meiyan Yang | Hanfang Chen | Qiaolan Huang | Zhuanbei Xie | Zekun Liu | Jumei Zhang | Yu Ding | Moutong Chen | Liang Xue | Qingping Wu | Juan Wang
<i>Vibrio parahaemolyticus</i> causes aquatic vibriosis. Its biofilm protects it from antibiotics; therefore, a new different method is needed to control <i>V. parahaemolyticus</i> for food safety. Phage therapy represents an alternative strategy to control biofilms. In this study, the lytic <i>Vibrio</i> phage vB_VpaP_FE11 (FE11) was isolated from the sewers of Guangzhou Huangsha Aquatic Market. Electron microscopy analysis revealed that FE11 has a typical podovirus morphology. Its optimal stability temperature and pH range were found to be 20–50 °C and 5–10 °C, respectively. It was completely inactivated following ultraviolet irradiation for 20 min. Its latent period is 10 min and burst size is 37 plaque forming units/cell. Its double-stranded DNA genome is 43,397 bp long, with a G + C content of 49.24% and 50 predicted protein-coding genes. As a lytic phage, FE11 not only prevented the formation of biofilms but also could destroy the formed biofilms effectively. Overall, phage vB_VpaP_FE11 is a potential biological control agent against <i>V. parahaemolyticus</i> and the biofilm it produces.
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