Germination and Culturability after UV Irradiation of <i>Metarhizium anisopliae</i> Native from Soils of Tropical Cattle Farms
2024
Miguel Ángel Alonso-Díaz | María de Lourdes Lozano-Velázquez | Iván Adrián García-Galicia | Agustín Fernández-Salas
The use of entomopathogenic fungi (EF) is a promising alternative for the control of <i>Rhipicephalus microplus</i>, an important tick affecting cattle globally. This study aimed to evaluate the effect of ultraviolet irradiation (UV) exposure on the percentage of conidia germination and number of colony-forming units of eight strains of <i>Metarhizium anisopliae</i> (MaV55, MaV35, MaV31, MaV25, MaV13, Ma08, MaV05, and MaV02). The UV (UV-A and UV-A+B) irradiation was carried out with an ultraviolet radiation emission lamp. The conidia of each strain were exposed to the UV irradiation treatments for 3 h. MaV25, MaV08, MaV05, MaV13, and MaV31 showed higher tolerance to UV-A radiation exposure, as assessed by conidia germination. UV-A+B radiation decreased the germination percentage of all the <i>M. anisopliae</i> strains. The eight evaluated strains showed good tolerance to UV-A radiation, as assessed by the development of colony-forming units (CFU). UV-A+B radiation did not significantly affect (<i>p</i> > 0.05) the count of the CFU of six of the <i>M. anisopliae</i> strains evaluated (MaV35, MaV13, MaV08, MaV05, MaV31, and MaV02). The novel findings of the UV-tolerant <i>M</i>. <i>anisopliae</i> strains may potentially improve the effectiveness of EF under environmental conditions. Integral research under real tropical conditions is advised to evaluate the effectiveness of the EF strains.
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