TYPING OF LEPTOSPIRA SPP. STRAINS BASED ON 16S rRNA
2016
Yu. V. Ostankova | A. V. Semenov | N. A. Stoyanova | N. K. Tokarevich | N. E. Lyubimova | O. A. Petrova | Yu. V. Ananina | E. M. Petrov
Aim. Comparative typing of Leptospira spp. strain collection based on analysis of 16S RNA fragment. Materials and methods. 2 pairs of primers were used for PCR, that jointly flank 1423 b.p. sized fragment. Sequences of Leptospira spp. strain 16S rRNA, presented in the international database, were used for phylogenetic analysis. Results. A high similarity, including interspecies, of the 16S fragment in Leptospira spp. strains was shown independently of the source, serovar and serogroup. Heterogeneity ofthe primary matrix, spontaneous mutations of hotspots and erroneous nucleotide couplings, characteristic for 16S sequence of pathogenic Leptospira spp. strains, are discussed. Molecular-genetic characteristic of certain reference Leptospira spp. strains by 16S sequence is obtained. Conclusion. Results of the studies give evidence on expedience of introduction into clinical practice of identification of Leptospira spp. by 16S sequence directly from the clinical material, that would allow to significantly reduce identification time, dismiss complex type-specific sera and other labor-intensive methods.
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