Antimicrobial Resistance Profile of <i>Planctomycetota</i> Isolated from Oyster Shell Biofilm: Ecological Relevance within the One Health Concept
2023
Bárbara Guedes | Ofélia Godinho | Sandra Quinteira | Olga Maria Lage
Background: <i>Planctomycetota</i> isolation in pure culture is still challenging with most of the reported data coming from molecular-based methods. Here, we intended to isolate <i>Planctomycetota</i> from the filter-feeder Pacific oyster <i>Magallana gigas</i>, extending the search to a not yet explored natural reservoir and to characterize their antimicrobial resistance phenotype. Methods: Oyster samples from different supermarkets and from a farm producer were subject to isolation in selective medium. Inoculation was performed from the shell biofilm and after an enrichment of the edible content. Results: <i>Planctomycetota</i> isolates (<i>n</i> = 65) were only obtained from the shell biofilm with four different species identified: <i>Rhodopirellula baltica</i> (<i>n</i> = 62), <i>Rhodopirellula rubra</i> (<i>n</i> = 1), <i>Rhodopirellula heiligendammensis</i> (<i>n</i> = 1) and <i>Gimesia chilikensis</i> (<i>n</i> = 1). This study reports the first association of <i>Planctomycetota</i> members with oysters and the first description of <i>R. heiligendammensis</i> in Portugal. Moreover, <i>R. rubra</i>, originally identified in Portugal, was isolated from oysters of French origin. Antibiotic susceptibility testing, conducted in strains belonging to two species never assayed before revealed multidrug resistance phenotypes with bacteria showing resistance to several classes of clinically relevant antibiotics (e.g., β-lactams and aminoglycosides). Conclusion: The ecological role and impact of <i>Planctomycetota</i> on oyster holobiont and, ultimately, in public health, under the One Health concept, is discussed.
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