<i>Rhododendron chrysanthum</i>’s Primary Metabolites Are Converted to Phenolics More Quickly When Exposed to UV-B Radiation
2023
Fushuai Gong | Wang Yu | Qingpan Zeng | Jiawei Dong | Kun Cao | Hongwei Xu | Xiaofu Zhou
The plant defense system is immediately triggered by UV-B irradiation, particularly the production of metabolites and enzymes involved in the UV-B response. Although substantial research on UV-B-related molecular responses in <i>Arabidopsis</i> has been conducted, comparatively few studies have examined the precise consequences of direct UV-B treatment on <i>R. chrysanthum</i>. The ultra-high-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) methodology and TMT quantitative proteomics are used in this study to describe the metabolic response of <i>R. chrysanthum</i> to UV-B radiation and annotate the response mechanism of the primary metabolism and phenolic metabolism of <i>R. chrysanthum</i>. The outcomes demonstrated that following UV-B radiation, the primary metabolites (L-phenylalanine and D-lactose*) underwent considerable changes to varying degrees. This gives a solid theoretical foundation for investigating the use of precursor substances, such as phenylalanine, to aid plants in overcoming abiotic stressors. The external application of ABA produced a considerable increase in the phenolic content and improved the plants’ resistance to UV-B damage. Our hypothesis is that externally applied ABA may work in concert with UV-B to facilitate the transformation of primary metabolites into phenolic compounds. This hypothesis offers a framework for investigating how ABA can increase a plant’s phenolic content in order to help the plant withstand abiotic stressors. Overall, this study revealed alterations and mechanisms of primary and secondary metabolic strategies in response to UV-B radiation.
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