Production of enterotoxins of Staphylococcus spp. isolated from samples of sheep milk

<span style="font-size:10.0pt;font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;mso-fareast-font-family:&quot;MS Mincho&quot;;mso-ansi-language: EN-GB;mso-fareast-language:SK;mso-bidi-language:AR-SA" lang="EN-GB">In our study was followed occurrence of mastitis in herd of 430 sheep of breed zoslachtena valaska with hand milking technology examined two times during one lactation season. Individual examination consisted from clinical examination of udder and microbiological examination of milk samples. By PCR was determined presence of genes coding production of enterotoxins, and by ELISA methods production individual types of enterotoxins. From individual forms of mastitis were frequently detected subacute (6.7%), subclinical (5.7%) and acute (2.9%). The coagulase-negative staphylococci (CNS) were identified in 102 (65.4%) from all 156 positive isolates. The CNS and <em>S. aureus</em> caused subacute (5.1%), subclinical (3.9%) and acute (2.4%) forms of mastitis. </span><span style="font-size:10.0pt;font-family:&quot;Times New Roman&quot;,&quot;serif&quot;;mso-fareast-font-family: &quot;MS Mincho&quot;;mso-ansi-language:SK;mso-fareast-language:SK;mso-bidi-language: AR-SA">The most frequently isolated were <em>S. epidermidis</em>, followed by <em>S. chromogenes </em>and<em> S. xylosus </em>from ewes with subacute and subclinical mastitis. From acute and chronical forms of mastitis were&nbsp; predominantly isolated <em>S. aureus</em>, <em>S. uberi</em>s and <em>S. epidermidis</em>. </span><span style="font-size:10.0pt;font-family:&quot;Times New Roman&quot;,&quot;serif&quot;;mso-fareast-font-family: &quot;MS Mincho&quot;;mso-ansi-language:EN-GB;mso-fareast-language:SK;mso-bidi-language: AR-SA" lang="EN-GB">The production of staphylococcal enterotoxins (SE) - SEA, SEB, SEC,&nbsp;SED and the presence of genes <em>sec</em> (3), <em>sea</em> (2), s<em>eb</em> (2) and <em>sed</em> (2) were determined in <em>S. aureus</em>, <em>S. epidermidis</em>, <em>S. schleiferi</em> and&nbsp;<em>S. chromogenes</em>, respectively. The results suggested on the high occurrence (12.4%) of subacute and subclinical forms. Confirmed production of enterotoxins and presence of genes coding their production present a risk for human health and decreased a quality of milk and products from sheep&acute;s milk.</span>