Characterization and antioxidant activity of semi-purified extracts and pure delphinidin-glycosides from eggplant peel (Solanum melongena L.)
2016
Pier Carlo Braga | Roberto Lo Scalzo | Monica Dal Sasso | Norma Lattuada | Valentina Greco | Marta Fibiani
Eggplant peels containing delphinidin-3-rutinoside or delphinidin-3-(p-coumaroylrutinoside)-5-glucoside (nasunin) were compared and characterized for antioxidant properties, using three cell-free assays with electron paramagnetic resonance (EPR) and two biological assays on human neutrophils with luminol amplified chemiluminescence (LACL). Antioxidant solutions were made with semi-purified extracts depleted in chlorogenic acid or with purified anthocyanin crystals. The semi-purified nasunin extract was higher in scavenging capacity than delphinidin-3-rutinoside in four assays (superoxide anion, hydroxyl radical, LACL, and Fremy's salt). Nasunin crystals showed higher antioxidant indices in Fremy's salt and LACL assays, with no detectable activity against the hydroxyl radical. The EC50 against free radicals was 0.4–20.6 nmol/mL in semi-purified extracts, and 1.7–73.6 nmol/mL for anthocyanin crystals. The higher activity of semi-purified extracts than crystals was attributed to other antioxidants, such as ascorbic acid, glutathione and biogenic amines, thus representing a percentage close to 30% of the total antioxidant content of eggplant peels.
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