反相高效液相色谱法同时测定植物油中8种维生素E异构体Simultaneous determination of eight vitamin E isomers in vegetable oils by RP-HPLC
2022
武利梅1，吴丽华1,2，赵晶晶2，吴晓霞1，李建科1WU Limei1, WU Lihua1,2, ZHAO Jingjing2, WU Xiaoxia1, LI Jianke1
为准确、快速地测定植物油中8种维生素E异构体，建立了均质快速提取法结合反相高效液相色谱法同时测定植物油中8种维生素E异构体的方法，并采用该方法对14种常见植物油进行测定。结果表明：与传统的皂化法和固相萃取法比较，均质快速提取法前处理快速简便，测定准确度和精密度高，重现性好；8种维生素E异构体在15 min内可以实现良好分离；在0.5~10 μg/mL质量浓度范围内8种维生素E异构体线性关系良好(R2＞0.999)，检出限为0.02~0.05 μg/mL，定量限为0.07~0.17 μg/mL，相对标准偏差在2.48%~5.79%之间，加标回收率在90.00%~110.00%之间；不同食用植物油中生育酚和生育三烯酚的种类和含量存在明显差异。建立的均质快速提取法结合反相高效液相色谱法适用于植物油中8种维生素E异构体的同时测定。 In order to accurately and quickly determine eight vitamin E isomers in vegetable oils，a method for simultaneous determination of eight vitamin E isomers in vegetable oils by homogeneous rapid extraction combined with RP-HPLC was established, and the method was used to determine 14 common vegetable oils. The results showed that compared with the conventional saponification method and solid-phase extraction method, the homogeneous rapid extraction method was fast and simple, with high accuracy, precision and good reproducibility. Eight kinds of vitamin E isomers could be well separated within 15 min. The linearities of eight vitamin E isomers were well in the range of 0.5-10 μg/mL(R2>0.999). The detection limits, quantitation limits, relative standard deviations and spiked recoveries were 0.02-0.05 μg/mL, 0.07-0.17 μg/mL, 2.48%-5.79% and 90.00%-110.00%, respectively. There were obvious differences in the types and contents of tocopherols and tocotrienols in different edible vegetable oils. The established homogeneous rapid extraction combined with RP-HPLC is suitable for the simultaneous determination of eight vitamin E isomers in vegetable oils.
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