Determination of phosphinothricin minimum lethal dose in banana Grande Naine (<em>Musa</em> AAA) transformants selection
2006
Idalmis Bermúdez-Caraballoso | Rafael Gómez-Kosky | Maritza Reyes | Borys Chong
The present work had the objective of determining the minimum lethal doses of the selective agent phosphineotricine, to establish the adequate conditions for the selection of genetically transformed cellular embryogenic aggregates of banana cultivar Grande Naine. Schenk and Hildebrandt culture medium, modified by Bieberach, was used , adding different concentrations of the selective agent at (0, 3, 6, 9 and 12 mg. l-1). The evaluation of the survival of the explants was carried out visually to the 30 days of culture using the scale elaborated in this work, as well as the cellular vitality for staining with Diacetate of Fluoresceine (DAF). The concentrations of the selective agent used, were the cause of the plant cells death. This effect was increased when increasing the concentration and the time of exposition from the vegetable material to the culture medium with the selective agent (increase of the toxicity). The minimal lethal doses selected was 6 mg.l-1 because the total necrosis in the banana cells and death of the tissue produced when was observed to the microscope the vitality of the cellular aggregates. Key words: cellular aggregates, genetic transformation, selection agents
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