Analysis of anthraquinone pigments of Pyrenophora tritici-repentis (Died.) Drechs
2013
Piotr Kachlicki | Wojciech Wakuliński
Pyrenophora tritici-repentis, anamorph Drechslera tritici-repentis, strains used in this study represented three phenotypic classes of this fungus: the wild type, strains unable to produce melanins - mel(-), and intermediate strains. Using an HPLC system with a reversed phase C18 column synthesis of anthraquinone pigments by strains belonging to all three classes could be detected. According to the 1H NMR spectra analysis the major compounds produced by P.tritici-repentis are catenarin, helminthosporin, islandicin, emodin, ω-hydroxy-islandicin, ω-hydroxy-catenarin and ω-hydroxy-helminthosporin. However, amounts of these compounds produced by the wild type were up to 350 times lower than those observed for the mel(-) forms. Some differences in profiles of these compounds produced by different strains were also observed.
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