In vitro establishment of Jacaratia spinosa (Aubl.) ADC / <br> Estabelecimento in vitro de Jacaratia spinosa (Aubl.) ADC

One of the problems faced in the daily life of the agricultural worker is the difficulty in the handle of the big packing bottles of agrochemicals, because they are normally very big, heavy and the handles are inefficient. Thinking about one way of making them more practical and useful the bottles manufactured by the company Cimplast Ltda and used by the industry Milênia Agro Ciência S.A. had been analyzed so that later, based in already carried trough studies in design, packing, ergonomics, anatomy and usability, a guide can be produce destined to the industries that produce this type of packing, so, by means of theoretical support , these companies can produce packing bottles that could adapt better to the user or could reformulate the existing models. For the production of the content of the guide some bibliographical references and technical norms directed to these packing bottles had been overcome. Later a research of field with the potential users was carried out, which it could confirm or refute the referring hypotheses to the usability raised initially. After the evaluation of the ergonomic problems presents in these packing bottles, tables of contents for better illustrate the research had been produced and, later, the data had been questioned based in the theoretical referential. The guide, which is the final product of this work, will bring important and scientifically based information, with simple and practical language, aiming to facilitate for the industries the process of production of the packing bottles, therefore although the data contained in it is available in literatures the companies make use of little time for research.<p><p>One of the problems faced in the daily life of the agricultural worker is the difficulty in the handle of the big packing bottles of agrochemicals, because they are normally very big, heavy and the handles are inefficient. Thinking about one way of making them more practical and useful the bottles manufactured by the company Cimplast Ltda and used by the industry Milênia Agro Ciência S.A. had been analyzed so that later, based in already carried trough studies in design, packing, ergonomics, anatomy and usability, a guide can be produce destined to the industries that produce this type of packing, so, by means of theoretical support , these companies can produce packing bottles that could adapt better to the user or could reformulate the existing models. For the production of the content of the guide some bibliographical references and technical norms directed to these packing bottles had been overcome. Later a research of field with the potential users was carried out, which it could confirm or refute the referring hypotheses to the usability raised initially. After the evaluation of the ergonomic problems presents in these packing bottles, tables of contents for better illustrate the research had been produced and, later, the data had been questioned based in the theoretical referential. The guide, which is the final product of this work, will bring important and scientifically based information, with simple and practical language, aiming to facilitate for the industries the process of production of the packing bottles, therefore although the data contained in it is available in literatures the companies make use of little time for research.<p><p>Com o objetivo de desenvolver um protocolo para o estabelecimento in vitro de Jacaratia spinosa a partir de diferentes concentrações de 6-benzilaminopurina (6-BA), foram utilizados como explantes, ápices caulinares de plantas provenientes do campo, desinfestados com solução de Saniagri® 33% v/v, durante 15 minutos e posteriormente pulverizados com uma solução de hipoclorito de sódio 1% v/v. O meio utilizado foi o Murashige e Skoog com a concentração de sacarose reduzida para 5g L-1. As concentrações de 6-BA foram: 0,0 mg L-1, 0,5 mg L-1, 1,0 mg L-1, 1,5 mg L-1, 2,0 mg L-1. O delineamento experimental usado foi o inteiramente casualizado, com 10 ápices por concentração de 6-BA. As avaliações visuais das freqüências de sobrevivência dos ápices e número de brotações laterais foram feitas semanalmente, por 90 dias, sendo os resultados em porcentagem submetidos à análise regressão e ao teste do Quiquadrado para várias proporções a 1% de significância. A porcentagem de descontaminação variou entre 60% e 80%. A curva de regressão sugere que a concentração zero apresentou sobrevivência superior às demais, porém o teste do Qui-quadrado mostra que as freqüências variaram de 41,7% até 58,3%, não havendo diferença estatística entre as concentrações de 6-BA. Não foi observada durante os 90 dias, a emissão de brotações laterais nos explantes, sendo necessários novos estudos com outras concentrações de fitoreguladores.