MOLECULAR CHARACTERIZATION OF SOME SEEDED TYPE DATE PALM TREES IN EGYPT
2018
A. Abu-Afifeh | Neima Al-Sanosy | Heba Ibrahim | Kh Soliman
In the present study, molecular fingerprinting among selected ten seeded type date palm trees growing at Al Dakhlah Oasis, New Valley Governorate in Egypt was carried out, in this approach 10 RAPD and 10 ISSR primers were used. In RAPD markers, primers 1, 3, 4, and 6 showed the highest level of polymorphism with number of polymorphic amplicons of 100%. While primers 7 and 8 resulted in the lowest number of polymorphic bands with polymorphism level of 62% and 63%, respectively. The similarity between Acsad-Dakhla 6 and 7 (0.76%) was observed as maximum similarity. While, Acsad-Dakhla 5 exhibited a minimum degree of similarity with all the seeded type date palm trees ranging from 33% to 49%. According to ISSR, highest polymorphism was generated by primers 8 and 9 (100%). While primers 4 and 2 produced the lowest polymorphism 50% and 55.6%, respectively. The highest similarity was observed between Acsad-Dakhla 8 and Acsad- Dakhla 9. Acsad-Dakhla 5 and Acsad-Dakhla 7 showed close relationship but Acsad-Dakhla 10 was at distant from all the seeded type date palm trees and did not lie in any subcluster. In combined analysis, the similarity between Acsad-Dakhla 6 and 7 were similar to Acsad-Dakhla 9 and 10 showed maximum similarity (85%). While, Acsad- Dakhla 2 and 5 exhibited a minimum degree of similarity with all the seeded type date palm trees (47%). A high genetic diversity among selected ten seeded type date palm trees was present.
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