In Vitro Screening and Field Performance of EMS-Treated Eggplants for the Selection of Shoot and Fruit Borer-Resistant Plants
2022
Md Ashraful Islam | Md Muntasir Bin Mohi Uddin | Md Golam Rasul | Md Ahsanul Haque Swapon | Minhaz Ahmed | Mehfuz Hasan
The shoot and fruit borer has asserted itself as a severe pest of eggplant. In vitro mutagenesis is an excellent method for producing mutants resistant to biotic and abiotic stresses. This study aimed to create eggplant mutants that were resistant to shoot and fruit borer infestation. In the Murashige and Skoog (MS) medium, two genotypes, including a landrace, were treated with varying amounts of EMS. Among the treatments, MS medium supplemented with 2% EMS demonstrated the most favorable effect. Explant performance dropped by up to 1.5% with an increase in EMS in the medium. The 2% EMS dose was appropriate for in vitro mutant eggplant development, but the higher dose was extremely damaging. EMS made several mutants sterile. Three landrace mutants were insect-resistant. Total phenols, leaf chlorophylls A and B, antioxidants, and solasodine were abundant in these genotypes. Correlation studies found a link between affected fruits and shoots and total phenols, antioxidants, and solasodine. Solasodine was shown to be related to chlorophylls A and B. The eggplant shoot and fruit borer infestation rate can be reduced by increasing these specific biochemical properties.
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