Artificial microRNA-Based RNA Interference and Specific Gene Silencing for Developing Insect Resistance in <i>Solanum lycopersicum</i>
2021
Mohammad Faisal | Eslam M. Abdel-Salam | Abdulrahman A. Alatar
RNA Interference (RNAi), which works against invading nucleic acids or modulates the expression of endogenous genes, is a natural eukaryotic regulating system, and it works by noncoding smaller RNA molecules. Plant-mediated gene silencing through RNAi can be used to develop plants with insect tolerance at transcriptional or post-transcriptional levels. In this study, we selected <i>Myzus persicae’s acetylcholinesterase</i> 1 gene (<i>Ace 1</i>) as a silencing target to develop transgenic <i>Solanum lycopersicum</i> L. plants’ resistance to aphids. An RNAi plasmid vector containing an artificial microRNA (amiRNA) sequence was engineered and successfully transformed into Jamila and Tomaland, two elite tomato cultivars. A northern blot analysis and PCR were carried out to check the efficacy of <i>Agrobacterium</i>-mediated transformation in T<sub>0</sub> transgenic plants. The quantitative PCR data showed a substantial downregulation of the <i>Ace 1</i> gene in aphids fed in clip cages on T<sub>1</sub> transgenic plants. Furthermore, there was a substantial drop in aphid colonies that were fed on T<sub>1</sub> transgenic plants of both the cultivars. These findings strongly suggest that transgenic plants that express amiRNA could be an important tool for engineering plants resistant to aphids and possibly for the prevention of viral disease in other plant-infested pests.
显示更多 [+] 显示较少 [-]