Optimization of Germination Process and Antioxidant Activity of Fagopyrum tataricum Based on Flavones Accumulation
2022
Yongliang BAI | Roumin LIN | Caiying WU | Weijun HE | Rong ZENG | Yu XIA
【Objective】With the total flavone content as an index, the germination process of Fagopyrum tataricum was optimized to explore the antioxidant activity in vitro.【Method】The effects of light intensity, germination temperature, germination time and relative humidity on the content of total flavones in F. tataricum were investigated by single factor experiment. On the basis of single factor, the germination process of F. tataricum was optimized by orthogonal experimental design. Changes of main flavones in F. tataricum before and after germination were compared. The evaluation indexes are based on the clearance rate of DPPH·free radical and hydroxyl radical, ferric reducing antioxidant power (FRAP) and ABTS+·free radical scavenging ability, the changes of its antioxidant activity in vitro of F. tataricum were studied.【Result】The germination conditions of F. tataricum were the optimal under light intensity of 7 500 lx, germination temperature of 25 ℃, germination time of 48 h and relative humidity of 80%. Under these conditions, the content of flavones in F. tataricum was 7.489%, an increase of 5.927 compared with that before germination. And the contents of hyperoside, rutin and 4'-O-glucosylvitexin were increased significantly. After germination, the DPPH·free radical clearance rate of F. tataricum was 54.39%, the ABTS+·free radical scavenging rate was 52.12%, and the FRAP reducing ability was 64.87%, which were significantly higher than those before germination.【Conclusion】Germination is beneficial to improve the flavone content and antioxidant activity of F. tataricum, which can be used for the development of functional products of F. tataricum and promote the high-value utilization of F. tataricum.
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