Lactic acid production on brewers' spent grain hydrolysate by lactobacillus Rhamnosus and Lactobacillus fermentum
2014
Pejin Jelena | Mojović Ljiljana | Kocić-Tanackov Sunčica | Radosavljević Miloš | Đukić-Vuković Aleksandra | Nikolić Svetlana
Brewers' spent grain (BSG) is the major by-product of the brewing industry, representing around 85% of the total by-products generated. Per 100 L of beer produced 20 kg of brewer's spent grain are obtained. BSG is a lignocellulosic material and due to its high content of protein and fibre, it can also serve as a raw material in biotechnology i.e. in lactic acid production. In this study brewer's spent grain hydrolysate was produced using optimal conditions. Hydrolysates were used for lactic acid fermentation by Lactobacillus rhamnosus ATCC 7469 and Lactobacillus fermentum PL-1. The effects of CaCO3 addition (2%) and different yeast extract content in hydrolysate on lactic acid yield were investigated. In fermentations with Lactobacillus rhamnosus ATCC 7469 high lactic acid yields were obtained (>95%) while in fermentations with Lactobacillus fermentum PL-1 lactic acid yields were low (<50%).
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