Determination of bean resistance to anthracnose pathogen – Colletotrichum lindemuthianum Br. et Cav
2020
A. M. Pashkevich | A. I. Tchaikovsky | N. V. Medved
Relevance. Currently, the range of vegetable crops has firmly entered the bean, the protein of which is absorbed by the human body by 87% or higher. The main factor limiting the cultivation of beans in Belarus is the lack of varieties of this crop that are resistant to mycological diseases, primarily Anthracnose.Materials and methods.The study included 34 samples of vegetable beans of mutant and hybrid origin obtained in the sector of legumes of vegetable crops of RUP "Institute of vegetable growing of the National Academy of Sciences of Belarus". To obtain a mutagenic effect, induced mutagenesis was used in the form of the action of a radioactive isotope 60Со and a neodymium laser. Hybrid combinations were obtained by intraspecific hybridization of mutant samples. For molecular genome testing, DNA was isolated from plant leaves before flowering, and its purification was performed by Fermentas using a Genomic DNA Purification Kit. The concentration of the resulting DNA solution was determined by a spectrophotometric method on a CARY 50 SCAN. A Thermo Hybaid RX2 amplifier was used for PCR. The annealing temperature of the primer was determined using the program "oligocalculator". The CV 2014 primer was used as a marker. resistant genotypes to Colletotrichum lindemuthianum Br. et Cav were at the band level of about 450 BP. Phytopathological assessment of plant infectivity was carried out on the basis of visual and instrumental methods, taking into account the reaction of plants and the intensity of the lesion, in the conditions of a natural infectious background in the phase of technical ripeness.Results. Seven samples of beans were identified that have genetic resistance to Anthracnose and show it in the conditions of a natural infectious background.
显示更多 [+] 显示较少 [-]