Cytomorphological and genetic indicators in the early ontogenesis of the wild and farmed broad whitefish (Coregonus nasus)
2023
Aleksandr Selyukov | Oksana Zhigileva | Leonid Shuman | Svetlana Selyukova | Vera Bogdanova
The qualitative and quantitative composition of primordial germ cells (PGCs) and the genetic variability of embryos of the Coregonus nasus from the Ob river basin and domesticated population breeding outside the natural areal was examined. In the broad whitefish, typical, binuclear, polymorphic nuclei cells, as well as PGCs represented primary gonocytes during embryogenesis in clusters. When hatching larvae PGCs mitoses were noted only in domesticated juveniles. The genetic variability of embryos of the broad whitefish was studied by polymerase chain reaction of inter simple sequence repeats (ISSR-PCR). The high polymorphism of the broad whitefish cultured population was revealed. The proportion of polymorphic bands was 89.4%, Nei's genetic diversity was 0.32. Reproduction of the domesticated broad whitefish did not affect the overall level of polymorphism of neutral DNA markers. These findings suggest the preservation of cytomorphological and genetic diversity in C. nasus embryo from different sources.
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