Evaluation of Trichoderma harzianum Rifai as resistance inducers against white rot Sclerotium rolfsii. Sacc in bean (Phaseolus vulgaris L.) under controlled conditions
2013
Jiménez María A | Arcia M | Asdrubal Arria | Catalina Ramis | Yreny de Faría
To evalúate the effect of Trichoderma harzianum as resistance inducers against white rot Sclerotium rolfsii. in bean (Phaseolus vulgaris L.) peroxidase enzymes (POX) and Final Disease Incidence (DI) an experiment was set, using the fungi in greenhouse conditions. The fungus inductor, under the commercial formulation was applied at a concentration of 1 x 107 conidia / mL to seeds of P. vulgaris cv. "Magdaleno" (cvM) and cv "Tacarigua" (cvT). Three days after the application was placed 3 sclerotia of the pathogen in the crown of the plants and placed in a completely randomized design with four treatments and four replications. Samples leaf to analysis of enzymes; from day 1 to day 7 post-inoculation were subjected. The separation of the isoenzymes was performed by polyacrylamide gel electrophoresis. The activity was induced POX 24hours after the application of T. harzianum and maintained until day seven. The enzyme activity by the pathogen was induced, fifth day in the cvT and second day at the cvM, revealing that potentially can be considered a resistant material. The results were consistent with those found in the assay under greenhouse conditions as DI decreased to 46% in the cvM demonstrating the importance of biochemical studies to determine the timing and frequency of application of the inductor, to design an integrated disease management along the crop.
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