METHODOLOGICAL RECOMMENDATIONS FOR TESTING AND ASSESSMENT OF EFFICIENCY OF MEDICATIONS AGAINST TRICHINELLOSIS AND HYMENOLEPIDOSIS IN LABORATORY MODEL
2019
I. A. Arkhipov | A. I. Varlamova | I. M. Odoevskaya
The purpose of the research is to characterize the methods for testing and assessment of efficiency of anthelmintics against trichinellosis and hymenolepidosis in laboratory model.Materials and methods. The literature and own research results have been analyzed as regards testing and efficiency assessment of anthelmintics against trichinellosis and hymenolepidosis in white mouse as a laboratory model weighting 14–16 g that was infected experimentally with Trichinella spiralis larvae in a dose of 200 larvae per specimen and with Hymenolepis nana eggs in a dose of 200 infective eggs per specimen. The medication efficiency was considered subject to the results of a small intestine autopsy on the 2nd and 4th days after the medications were injected to treat trichinellosis and hymenolepidosis in white mice accordingly.Results and discussion. A detailed description has been given for progressive methods to test medication and new chemical compound effect on nematode activity in white mice infected experimentally with T. spiralis, and on cestode activity in white mice infected experimentally with H. nana in a dose of 200 larvae and 100–200 eggs per specimen accordingly. In order to study effectiveness, the test medications were introduced into animals on the 3rd day in the trichinellosis case and on the 13th day in the hymenolepidosis case. Anthelmintic effectiveness was taken into consideration based on helminthologic autopsy of small intestines in animals on the 2nd and 4th days after the medications were given against T. spiralis и H. nana accordingly. The helminthes found in the experimental and control (uncured) animals were counted by a checkup analysis, namely, in comparison with the control animals.
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