Metabolic Deregulation of the Blood-Outer Retinal Barrier in Retinitis Pigmentosa
2019
Wei Wang | Ashwini Kini | Yekai Wang | Tingting Liu | Yao Chen | Eric Vukmanic | Douglas Emery | Yongqing Liu | Xiaoqin Lu | Lei Jin | San Joon Lee | Patrick Scott | Xiao Liu | Kevin Dean | Qingxian Lu | Enzo Fortuny | Robert James | Henry J. Kaplan | Jianhai Du | Douglas C. Dean
Summary: Retinitis pigmentosa (RP) initiates with diminished rod photoreceptor function, causing peripheral and night-time vision loss. However, subsequent loss of cone function and high-resolution daylight and color vision is most debilitating. Visual pigment-rich photoreceptor outer segments (OS) undergo phagocytosis by the retinal pigment epithelium (RPE), and the RPE also acts as a blood-outer retinal barrier transporting nutrients, including glucose, to photoreceptors. We provide evidence that contact between externalized phosphatidylserine (PS) on OS tips and apical RPE receptors activates Akt, linking phagocytosis with glucose transport to photoreceptors for new OS synthesis. As abundant mutant rod OS tips shorten in RP, Akt activation is lost, and onset of glucose metabolism in the RPE and diminished glucose transport combine to cause photoreceptor starvation and accompanying retinal metabolome changes. Subretinal injection of OS tip mimetics displaying PS restores Akt activation, glucose transport, and cone function in end-stage RP after rods are lost. : Wang et al. show that onset of glucose metabolism in the retinal pigment epithelium (RPE), which acts as the blood-outer retinal barrier, and inhibition of RPE glucose transport to photoreceptors combine to cause photoreceptor starvation and vision loss in retinitis pigmentosa. Keywords: retinitis pigmentosa, retinal pigment epithelium, photoreceptors, vision, metabolism, glucose transport
显示更多 [+] 显示较少 [-]