Inactivation of severe fever with thrombocytopenia syndrome virus for improved laboratory safety
2020
Toshihiko Harada | Shuetsu Fukushi | Takeshi Kurosu | Tomoki Yoshikawa | Masayuki Shimojima | Kiyoshi Tanabayashi | Masayuki Saijo
Summary: Severe fever with thrombocytopenia syndrome (SFTS), a tick-borne infectious disease with high mortality, is diagnosed by the serological testing of serum samples from patients in the acute and convalescent phases and/or by direct detection of the SFTS virus (SFTSV). Conventionally, heat and UV treatments have been used to inactivate the viruses present in serum samples before performing the serological assays. Here, we examined the inactivation conditions optimal for SFTSV-containing serum samples to ensure the safety of laboratory workers while maintaining the accuracy of serological assay results simultaneously. Heating human serum samples spiked with SFTSV to 60 °C for 30 min or exposing them to UV irradiation for 30 min, reduced the infectious virus titer below the limit of detection. SFTSV in sera from patients in the acute phase of SFTS was completely inactivated by heating to 60 °C for 30 min, followed by UV irradiation for 10 min. This inactivation procedure had minimal impact on the performance of SFTSV antibody detection tests. The data provided herein can serve as a guide for laboratory workers and researchers working with SFTS serological tests.
显示更多 [+] 显示较少 [-]