Development of Microsatellite Markers in <i>Cratylia mollis</i> and their Transferability to <i>C. argentea</i> (Fabaceae)
2013
M. Cristina López-Roberts | Luciano Paganucci De Queiroz | Cássio Van Den Berg
Premise of the study: This work aimed to develop microsatellite markers for <i>Cratylia mollis</i> as tools to assess its genetic diversity and structure and to evaluate their potential cross-amplification in related species. Methods and Results: Microsatellite markers were developed using a microsatellite-enriched library and an intersimple sequence repeat library. From a set of 19 markers, 12 microsatellite loci were polymorphic and presented considerable variation in allele number (2–11), expected heterozygosity (0.226–0.883), and polymorphism information content per locus (0.212– 0.870). Cross-amplification in <i>C. argentea</i> was successful in 16 loci, 12 of which were polymorphic (2–10 alleles). Conclusions: The polymorphism of this set of microsatellite markers for <i>C. mollis</i>, as well as their successful cross-amplification in <i>C. intermedia</i> and <i>C. bahiensis</i> and their transferability to <i>C. argentea</i>, supports their use in future comparative studies to understand the mechanism involved in population divergence and speciation in the genus.
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