Chemosystematic of Enterobacteriaceae Familia Obtained from Blood Cultures Based on Total Protein Profiles
2015
Sri Darmawati | Langkah Sembiring | Widya Asmara | Wayan T. Artama | Syaiful Anwar
<div>The purpose of this study was to determine the chemosystematic of 14 strains of bacteria in blood cultures from Semarang using 1 reference strain S. typhi NCTC 786, based on the total protein profi les with the similarity relationship analysis based on Simple Matching Coeffi cient (SSM) analysis and algorithm method</div><div>of unweighted pair group with averages (UPGMA) presented in a dendrogram. The results showed that the</div><div>chemosystematic based on the total protein profi les using SDS-PAGE method can classify the member of</div><div>bacterial strains of each species. The Clusters respectively consist of 4 strains of S. typhi (similarity: 89.7%),</div><div>2 strains of Ser. marcescens (similarity: 89.7%), two strains of E. coli, and one strain of Salmonella ssp, S. typhi NCTC 786 (similarity: 100%). Those three incorporated clusters had the similarity value of 75.3%. Those four strains of Ent. cloacae composed in one cluster (similarity: 100%) are incorporated in a cluster consisting of one strain of Kleb. pneumoniae (similarity: 92.9%). Both clusters were incorporated in a cluster consisting of S. typhi NCTC 786 (similarity: 67.9%).</div><div><br /></div><div>Key words: Enterobacteriaceae, chemosystematic, blood cultures, protein profile</div>
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