Immunomodulation and Antitumor Activities of Degraded Polysaccharide from Marine Microalgae Sarcinochrysis marina Geitler
2013
Liqin Sun | Ling Wang | Jing Li | Honghui Liu
The polysaccharide (SMP) from Sarcinochrysis marina Geitler were degraded using ascorbate and hydrogen peroxide in combination by ultrasonic assistant, and the degraded fractions were purified by gelchromatography. Then three different uniform fragments (SMP1, SMP2 and SMP3) were obtained with molecular weight of 452.79, 168.66 and 8.69 kDa. Their effects on peritoneal macrophage and spleen lymphocyte activation in vitro and the growth of mouse transplantable tumor S180 were measured. The results showed that the degraded fragments could evidently increased phagocytosis and proliferation of macrophage, stimulate NO emission to different extents, and promote propagate of lymphocytes. The immunocompetence had a direct proportion to molecular weight of the samples. The low molecular weight fraction (SMP3) had the strongest immunity activity. Different doses of samples could significantly inhibit the growth of S180 solid tumors, increase the thymus index and spleen index, and promote splenocyte proliferation of tumor-bearing mice. The effect is dose-dependent in a linear fashion. SMP2 had the strongest antitumor activity, and the tumor inhibition index of 200 mg/kg/day dose was 58.51%. All of above results indicated that the antitumor activity of SMP might be achieved by improving body immune response.
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