Determination of concentration of the pseudomycelles from fermentation broth via spectroscopy and Neubauer chamber
2020
Alessandra Suzin Bertan | Marco Aurélio Cremasco
The present work presents an alternative technique to obtain the concentration of pseudomycelles as number of pseudomycelles/sample volume, obtained by Neubauer chamber, as function of absorbance from spectroscopy. Samples of broth from fermentation via bacteria Streptomyces tsukubaensis were analyzed, making it possible to obtain a linear relationship between the number of pseudomycelles/ sample volume and absorbance, with determination coefficient of 0.995. In addition, a morphological analysis of the unfermented pseudomycelles was performed using scanning electron microscopy (SEM) at the magnifications of 20, 2000, 5000, 10000 and 20000 x. DOI: http://dx.doi.org/10.5281/zenodo.3987017
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