Fin clipping and genotyping embryonic zebrafish at 3 days post-fertilization

Robert N. Wilkinson; Stone Elworthy; Philip W. Ingham; Fredericus J.M. van Eeden

Fin clipping and genotyping embryonic zebrafish at 3 days post-fertilization

2017

Robert N. Wilkinson | Stone Elworthy | Philip W. Ingham | Fredericus J.M. van Eeden

Protocol Summary Here we describe a method for high-throughput genotyping of live larval zebrafish as early as 72 h post-fertilization (hpf). Importantly, this technique allows rapid and cost-effective PCR-based genotyping from very small fin biopsies, which regenerate as the embryo develops, thereby allowing researchers to select embryos with desired genotypes to be raised to adulthood.

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