Transcardiac Perfusion of the Mouse for Brain Tissue Dissection and Fixation

Jinyun Wu; Yuqi Cai; Xiaoyang Wu; Yue Ying; Yilin Tai; Miao He

Transcardiac Perfusion of the Mouse for Brain Tissue Dissection and Fixation

2021

Transcardiac perfusion with saline followed by 4% paraformaldehyde (PFA) is widely used to clear blood and preserve brain for immunostaining or in situ hybridization. PFA breaks into formaldehyde in solution, which cross-link protein and DNA molecules to preserve tissue and cell structure. Here we provide a step by step guide for performing this procedure in mouse.

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