Streptavidin Bead Pulldown Assay to Determine Protein Homooligomerization

Ting-Hai Xu; Yan Yan; Kaleeckal Harikumar; Laurence Miller; Karsten Melcher; H. Xu

Streptavidin Bead Pulldown Assay to Determine Protein Homooligomerization

2017

Pulldown assay is a conventional method to determine protein-protein interactions in vitro. Expressing a protein of interest with two different tags allows testing whether both versions can be captured via one of the two tags as homooligomeric complex. This protocol is based on streptavidin bead capture of a biotinylated protein and co-associated Flag-tagged protein using Streptavidin MagBeads.

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