Efficient Isolation of Influenza Specific CTLs

Prerak  Trivedi; Stuart  Mannering

Efficient Isolation of Influenza Specific CTLs

2016

Prerak Trivedi | Stuart Mannering

Human antigen-specific CD8+ T-cell clones are valuable tools for dissecting CD8+ T-cell responses against antigens derived from infectious agents, cancer and self antigens. Here we describe a protocol for isolating human antigen-specific CD8+ T cells. This protocol uses surface capture of IFNγ to identify antigen responsive cells that are then cloned by single-cell sorting. Here we use CD8+ T-cell responses to influenza matrix protein (MP) as an example, but this approach can be applied to any antigen specificity.

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