Transcriptome Analysis Reveals the Function of a G-Protein α Subunit Gene in the Growth and Development of <i>Pleurotus eryngii</i>
2023
Jixuan Cao | Meijing Sun | Mingming Yu | Yanfei Xu | Jiacheng Xie | Huangru Zhang | Jiayi Chen | Tao Xu | Xin Qian | Shujing Sun
<i>Pleurotus eryngii</i> is a commercially important edible fungus with high nutritional and economic value. However, few functional studies have examined key genes affecting the growth and development of <i>P. eryngii</i>. In this study, transformed strains, including over-expression (<i>PeGNAI</i>-OE) and RNA interference (<i>PeGNAI</i>-RNAi) lines, were constructed to elucidate the role of <i>GNAI</i> in <i>P. eryngii</i> growth. <i>GNAI</i> expression was found to affect the mycelial growth and the number of clamp connections. Moreover, the transformed strains were shown to have higher endogenous cAMP levels, thus affecting amylase and laccase activity. Fruiting experiments showed that <i>GNAI</i> expression revealed the formation of <i>P. eryngii</i> primordia and the number of buttons, while transcription analysis identified <i>GNAI</i> gene involvement in the growth and development of <i>P. eryngii</i>. Seven downstream genes regulated by <i>GNAI</i> were differentially expressed in <i>PeGNAI-OE</i> and <i>PeGNAI</i>-RNAi compared to wild type (WT). These genes may be related to mycelial growth and enzyme activity. They were involved in the MAPK signaling pathway, inositol phosphate metabolism, ascorbate, aldarate metabolism, and starch and sucrose metabolism. In summary, <i>GNAI</i> performs different physiological functions in regulating the growth and development of <i>P. eryngii</i>. Importantly, the molecular mechanisms of <i>GNAI</i> regulatory function are relatively complex and need further study.
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