Adenylsuccinate Synthetase MoADE12 Plays Important Roles in the Development and Pathogenicity of the Rice Blast Fungus
2022
Zhen Zhang | Zhongna Hao | Rongyao Chai | Haiping Qiu | Yanli Wang | Jiaoyu Wang | Guochang Sun
Purines are basic components of nucleotides in living organisms. In this study, we identified the ortholog of adenylosuccinate synthase <i>MoADE12</i> in <i>Magnaporthe oryzae</i> by screening for growth-defective T-DNA insertional mutants. Gene replacement was performed to investigate the biological role of <i>MoADE12</i>. <i>Δmoade12</i> mutants were adenine auxotrophs that failed to produce conidia, and showed reduced perithecia formation and pathogenicity. Moreover, the <i>Δmoade12</i> mutant was hypersensitive to Congo red and oxidants, indicating that <i>MoADE12</i> was required for cell wall integrity and oxidative stress resistance. Transcriptomic analysis identified the underlying mechanisms and indicated that several pathogenicity-related genes were regulated in the <i>Δmoade12</i> mutant. Therefore, our data suggest that the adenylosuccinate synthase <i>MoADE12</i> is involved in the de novo AMP biosynthesis pathway and is important for conidiation and pathogenicity in the rice blast fungus.
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