Ferric Chloride Controls Citrus Anthracnose by Inducing the Autophagy Activity of <i>Colletotrichum gloeosporioides</i>

<i>Colletotrichum gloeosporioides</i> causes citrus anthracnose, which seriously endangers the pre-harvest production and post-harvest storage of citrus due to its devastating effects on fruit quality, shelf life, and profits. However, although some chemical agents have been proven to effectively control this plant disease, little to no efforts have been made to identify effective and safe anti-anthracnose alternatives. Therefore, this study assessed and verified the inhibitory effect of ferric chloride (FeCl₃) against <i>C. gloeosporioides</i>. Our findings demonstrated that FeCl₃ could effectively inhibit <i>C. gloeosporioides</i> spore germination. After FeCl₃ treatment, the germination rate of the spores in the minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) groups decreased by 84.04% and 89.0%, respectively. Additionally, FeCl₃ could effectively inhibit the pathogenicity of <i>C. gloeosporioides</i> in vivo. Optical microscopy (OM) and scanning electron microscopy (SEM) analyses demonstrated the occurrence of wrinkled and atrophic mycelia. Moreover, FeCl₃ induced autophagosome formation in the test pathogen, as confirmed by transmission electron microscopy (TEM) and monodansylcadaverine (MDC) staining. Additionally, a positive correlation was identified between the FeCl₃ concentration and the damage rate of the fungal sporophyte cell membrane, as the staining rates of the control (untreated), 1/2 MIC, and MIC FeCl₃ treatment groups were 1.87%, 6.52%, and 18.15%, respectively. Furthermore, the ROS content in sporophyte cells increased by 3.6%, 29.27%, and 52.33% in the control, 1/2 MIC, and MIC FeCl₃ groups, respectively. Therefore, FeCl₃ could reduce the virulence and pathogenicity of <i>C. gloeosporioides</i>. Finally, FeCl₃-handled citrus fruit exhibited similar physiological qualities to water-handled fruit. The results show that FeCl₃ may prove to be a good substitute for the treatment of citrus anthracnose in the future.