In-depth human plasma proteome analysis captures tissue proteins and transfer of protein variants across the placenta
2019
Maria Pernemalm | AnnSofi Sandberg | Yafeng Zhu | Jorrit Boekel | Davide Tamburro | Jochen M Schwenk | Albin Björk | Marie Wahren-Herlenius | Hanna Åmark | Claes-Göran Östenson | Magnus Westgren | Janne Lehtiö
Here, we present a method for in-depth human plasma proteome analysis based on high-resolution isoelectric focusing HiRIEF LC-MS/MS, demonstrating high proteome coverage, reproducibility and the potential for liquid biopsy protein profiling. By integrating genomic sequence information to the MS-based plasma proteome analysis, we enable detection of single amino acid variants and for the first time demonstrate transfer of multiple protein variants between mother and fetus across the placenta. We further show that our method has the ability to detect both low abundance tissue-annotated proteins and phosphorylated proteins in plasma, as well as quantitate differences in plasma proteomes between the mother and the newborn as well as changes related to pregnancy.
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