Interactome of Two Diverse RNA Granules Links mRNA Localization to Translational Repression in Neurons
2013
Renate Fritzsche | Daniela Karra | Keiryn L. Bennett | Foong yee Ang | Jacki E. Heraud-Farlow | Marco Tolino | Michael Doyle | Karl E. Bauer | Sabine Thomas | Melanie Planyavsky | Eric Arn | Anetta Bakosova | Kerstin Jungwirth | Alexandra Hörmann | Zsofia Palfi | Julia Sandholzer | Martina Schwarz | Paolo Macchi | Jacques Colinge | Giulio Superti-Furga | Michael A. Kiebler
Transport of RNAs to dendrites occurs in neuronal RNA granules, which allows local synthesis of specific proteins at active synapses on demand, thereby contributing to learning and memory. To gain insight into the machinery controlling dendritic mRNA localization and translation, we established a stringent protocol to biochemically purify RNA granules from rat brain. Here, we identified a specific set of interactors for two RNA-binding proteins that are known components of neuronal RNA granules, Barentsz and Staufen2. First, neuronal RNA granules are much more heterogeneous than previously anticipated, sharing only a third of the identified proteins. Second, dendritically localized mRNAs, e.g., Arc and CaMKIIα, associate selectively with distinct RNA granules. Third, our work identifies a series of factors with known roles in RNA localization, translational control, and RNA quality control that are likely to keep localized transcripts in a translationally repressed state, often in distinct types of RNPs.
显示更多 [+] 显示较少 [-]