Effect of KISS1 gene variants (rs372790354 G>A and rs4889 G>A) on kisspeptin in patients with polycystic ovary syndrome in Iraq.
2022
Noor J. Al-Musawi | Suhayr Aesa Al- Qaysi | Suha J. Witwit
Polycystic ovary syndrome (PCOS) is a heterogeneous genetic disorder categorized by hyperandrogenism that affects early reproductive age in females, KISS1 has play role in regulating the hypothalamic-pituitary-gonad axis, it plays a key role in human reproductive function. In patients with polycystic ovarian syndrome, imbalance-of-function mutations in this gene have been found often. blood samples were collected from 120 patients (60 control are divided into 30 normal weight and 30 obese) and (60 PCOS) females are divided into 30 normal weight and 30 obese). DNA was extracted and genotyped for KISS1 variants by HRM-PCR and measured level of kisspeptin by ELIAS, while LH, FSH, DHEA and free testosterone by CLIA. The value of LH, Testosterone, DHEA-S and kisspeptin is elevated in the patient group, while the decline of FSH in serum level patients value, rs372790354 G > A and rs4889 G>A was associated with PCOS in dominant, recessive, co-dominant (P-value< 0.05), rs37279054 AA was not found the effect of obese group and linked with normal weight PCOS put present study no effect on the parameters, rs4889 GG/GA was the effect on all subgroups except the genotype GA not effected on obese female, the highly significant ( P-valueA may be associated with the pathophysiology of PCOS and lead to increase serum level of LH that due to hyperandrogenism. [ J Adv Biotechnol Exp Ther 2022; 5(3.000): 562-576]
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