T4 DNA polymerase improves the efficiency of multiple site-directed mutagenesis
2016
Wei Guo | Shuhui Sun | Binghua Xie | Xiao-Jing Zhu | Zhong-Min Dai
Site-directed mutagenesis (SDM) is a useful tool to study the functions of regulatory sequences of DNA and RNA, and the structures and functions of proteins. Numerous methods have been developed for either single or multiple SDM (MSDM). However, MSDM is sometimes difficult. Here we demonstrated that T4 DNA polymerase greatly enhanced the efficiency of MSDM. Moreover, we have also showed that it is efficient to clone multiple specific mutation-containing sequences simultaneously.
显示更多 [+] 显示较少 [-]书目信息
出版者
Taylor & Francis Group
其它主题
Complementary annealing mediated by exonuclease; Multiple site-directed mutagenesis; Dna assembly; T4 dna polymerase
语言
英语
2024-12-12
2026-02-03
DOAJ