Genetic structure analysis of leaf rust resistant triticale accessions from the VIR collection using gliadin patterns

The gliadin banding patterns of important accessions from the collection of the N. I. Vavilov All‑Russian Institute of Plant Genetic Resources (VIR) registered in the form of “protein formulas” provide reliable information for the preparation of a “protein passport” for each accession and is convenient for storage and computer processing. It helps to control originality and integrity of accessions during regeneration and their use in breeding. The study involved 17 triticale accessions resistant to leaf rust. The analysis was carried out on single grains of the original accession (a sample of 13–26 kernels) according to the standard protocol adopted by VIR and approved by the International Seed Testing Association (ISTA). The gliadin electrophoretic banding patterns of triticale accessions were registered in the form of “protein formulas”; polymorphism of each accession and genetic diversity within the collection were estimated, and genetic structure of accessions was identified based on the marker protein components. A large variety of the revealed genotypes opens a possibility to identify accessions that combine resistance with other useful traits. Stable and polymorphic accessions including from 2 to 7 biotypes were found. The discovery of interbiotype hybrids and recombinant genotypes in the composition of some polymorphic accessions indicates the instability of their genetic structure and the ongoing formation process. This is due to the heterogeneity of the original parental forms, the tendency to cross‑pollination and insufficiently thorough selection. The data on the triticale genotypic structure can be used in introgressive breeding to control the transfer of rye genetic material to wheat varieties in order to increase their immunity and resistance to adverse factors.