Improved efficiency of doubled haploid generation in hexaploid triticale by <it>in vitro</it> chromosome doubling
2012
Würschum Tobias | Tucker Matthew R | Reif Jochen C | Maurer Hans
<p>Abstract</p> <p>Background</p> <p>Doubled haploid production is a key technology in triticale research and breeding. A critical component of this method depends on chromosome doubling, which is traditionally achieved by <it>in vivo</it> treatment of seedlings with colchicine.</p> <p>Results</p> <p>In this study we investigated the applicability of an <it>in vitro</it> approach for chromosome doubling based on microspore culture. Our results show a pronounced increase in the proportion of doubled haploid triticale plants compared to the spontaneous doubling rate, but also compared to the doubling obtained by the standard <it>in vivo</it> approach. In addition, the frequency of plants surviving from culture medium to maturity is also much higher for the <it>in vitro</it> approach. Colchicine concentrations of 1 mM for 24 h or 0.3 mM applied for 48 or 72 h during the first hours of microspore culture performed best.</p> <p>Conclusions</p> <p>Our results suggest that for triticale, <it>in vitro</it> chromosome doubling is a promising alternative to the <it>in vivo</it> approach.</p>
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