Effects of Jasmonate on Ethylene Function during the Development of Tomato Stamens
2019
Ramona Schubert | Stephan Grunewald | Lea von Sivers | Bettina Hause
The phenotype of the tomato mutant <i>jasmonate-insensitive1-1</i> (<i>jai1-1</i>) mutated in the JA-Ile co-receptor COI1 demonstrates JA function in flower development, since it is female-sterile. In addition, <i>jai1-1</i> exhibits a premature anther dehydration and pollen release, being in contrast to a delayed anther dehiscence in the JA-insensitive Arabidopsis mutant <i>coi1-1</i>. The double mutant <i>jai1-1 Never ripe</i> (<i>jai1-1 Nr</i>), which is in addition insensitive to ethylene (ET), showed a rescue of the <i>jai1-1</i> phenotype regarding pollen release. This suggests that JA inhibits a premature rise in ET to prevent premature stamen desiccation. To elucidate the interplay of JA and ET in more detail, stamen development in <i>jai1-1 Nr</i> was compared to wild type, <i>jai1-1</i> and <i>Nr</i> regarding water content, pollen vitality, hormone levels, and accumulation of phenylpropanoids and transcripts encoding known JA- and ET-regulated genes. For the latter, RT-qPCR based on nanofluidic arrays was employed. The data showed that additional prominent phenotypic features of <i>jai1-1</i>, such as diminished water content and pollen vitality, and accumulation of phenylpropanoids were at least partially rescued by the ET-insensitivity. Hormone levels and accumulation of transcripts were not affected. The data revealed that strictly JA-regulated processes cannot be rescued by ET-insensitivity, thereby emphasizing a rather minor role of ET in JA-regulated stamen development.
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