Phytochemical and In Vitro Genotoxicity Studies of Standardized <i>Ficus deltoidea</i> var. <i>kunstleri</i> Aqueous Extract
2021
Hussin Muhammad | Maizatul Hasyima Omar | Elda Nurafnie Ibnu Rasid | Shazlan Noor Suhaimi | Farah Huda Mohkiar | Lau Mei Siu | Norizah Awang
The present study was carried out to assess the genotoxicity potential of <i>Ficus deltoidea</i> var. <i>kunstleri</i> aqueous extract (FDAE) using standard in vitro assays. The DNA damage of V79B cells was measured using the alkaline comet assay treated at 0.1 mg/mL (IC10) and 0.3 mg/mL (IC25) of FDAE together with positive and negative controls. For in vitro micronucleus assay, the V79B cells were treated with FDAE at five different concentrations (5, 2.5, 1.25, 0.625, and 0.3125 mg/mL) with and without S9 mixture. The bacteria reverse mutation assay of FDAE was performed on <i>Salmonella typhimurium</i> strains TA98, 100, 1535, 1537, and <i>Escherichia coli</i> strain WP2<i>uvrA</i> using pre-incubation method in the presence or in the absence of an extrinsic metabolic system (S9 mixture). FDAE at 0.1 and 0.3 mg/mL significantly increased DNA damage in both comet tail and tail moment (<i>p</i> < 0.05). No significant changes were detected in the number of micronucleated cell when compared to control. Tested at the doses up to 5000 µg/plate, the FDAE did not increase the number of revertant colonies for all strains. In conclusion, further investigation needs to be conducted in animal model to confirm the non-genotoxicity activities of FDAE.
显示更多 [+] 显示较少 [-]