<i>Koliella bifissiva</i> sp. nov (Chlorellaceae, Chlorophyta) and Analysis of Its Organelle Genomes
2024
Huiyin Song | Hai Peng | Zhiwei Fang | Baolong Zhang | Zhaolu Zhu | Zilan Xiao | Guoxiang Liu | Yuxin Hu
Chlorellacean members are common in aquatic or subaerial habitats, and many of them have significant economic value. Taxonomic reports and organelle genome data for the <i>Nannochloris</i> clade, an important subgroup within this family, are limited, hindering the understanding and exploitation of this clade. In this study, a fusiform-celled strain, FACHB-3607, was isolated from a pond in China. Through examination of morphological characteristics and phylogenetic analyses of rbcL, 18S rDNA, and ITS, it was identified as a new species within the <i>Nannochloris</i> clade, named <i>Koliella bifissiva</i> sp. nov. In addition, this study provided a first insight into the organellar genomes of the genus <i>Koliella</i>. The <i>K. bifissiva</i> chloroplast had a 99.8 kb genome, and the mitochondrion had a 40.8 kb genome, which are moderate sizes within the <i>Nannochloris</i> clade. Phylogenomic analysis showed that <i>K. bifissiva</i> is most closely related to <i>Nannochloris</i> sp. “desiccata”, followed by <i>Marvania</i>. In contrast, <i>Picochlorum</i> was the most distantly related species. The organelle genomes of the <i>Nannochloris</i> clade display dynamic evolution, reflected in variations in genome size, gene content and order, and selection pressure. This research enhances our knowledge of species diversity and evolutionary history in the <i>Nannochloris</i> clade.
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