Disruption of a DUF247 Containing Protein Alters Cell Wall Polysaccharides and Reduces Growth in <i>Arabidopsis</i>
2023
Pitchaporn Wannitikul | Pakorn Wattana-Amorn | Sukhita Sathitnaitham | Jenjira Sakulkoo | Anongpat Suttangkakul | Passorn Wonnapinij | George W. Bassel | Rachael Simister | Leonardo D. Gomez | Supachai Vuttipongchaikij
Plant cell wall biosynthesis is a complex process that requires proteins and enzymes from glycan synthesis to wall assembly. We show that disruption of <i>At3g50120</i> (<i>DUF247-1</i>), a member of the <i>DUF247</i> multigene family containing 28 genes in <i>Arabidopsis</i>, results in alterations to the structure and composition of cell wall polysaccharides and reduced growth and plant size. An ELISA using cell wall antibodies shows that the mutants also exhibit ~50% reductions in xyloglucan (XyG), glucuronoxylan (GX) and heteromannan (HM) epitopes in the NaOH fraction and ~50% increases in homogalacturonan (HG) epitopes in the CDTA fraction. Furthermore, the polymer sizes of XyGs and GXs are reduced with concomitant increases in short-chain polymers, while those of HGs and mHGs are slightly increased. Complementation using 35S:<i>DUF247-1</i> partially recovers the XyG and HG content, but not those of GX and HM, suggesting that DUF247-1 is more closely associated with XyGs and HGs. <i>DUF247-1</i> is expressed throughout <i>Arabidopsis</i>, particularly in vascular and developing tissues, and its disruption affects the expression of other gene members, indicating a regulatory control role within the gene family. Our results demonstrate that DUF247-1 is required for normal cell wall composition and structure and <i>Arabidopsis</i> growth.
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