Development and Characterization of EST-SSR Markers for the <i>Solidago virgaurea</i> Complex (Asteraceae) in the Japanese Archipelago
2014
Shota Sakaguchi | Motomi Ito
Premise of the study: We developed simple sequence repeat (SSR) markers from expressed sequence tags (ESTs) for the <i>Solidago virgaurea</i> complex, an ecologically and morphologically diverse species complex in the Japanese archipelago, to elucidate population genetic structure and examine taxonomic boundaries. Methods and Results: Utilizing the RNA sequencing data obtained by next-generation sequencing techniques, 15 polymorphic EST-SSR markers with three to 14 alleles were developed, most of which were transferable to different <i>Solidago</i> species native to Eurasia and North America. Conclusions: The EST-SSR markers developed in this study may be useful for elucidating the population structure and taxonomic delimitation of the species complex, as well as for investigating the population genetics and reproductive ecology of <i>Solidago</i> species.
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