Influence of gastrointestinal digests of glycated whey protein on the hormone secretion of enteroendocrine cells
2023
Kexin Zhang | Yuxuan Wang | Bulei Sheng | Di Zhao
The present research used two α-dicarbonyl compounds, glyoxal and methylglyoxal and whey protein as subjects, and evaluated the alterations of physicochemical properties, structure, and digestibility of whey protein throughout the glycation process. The STC-1 cells were used as a model to investigate the implications of glycation on the protein nutrition sensing of enteroendocrine cells. Results showed that glycation substantially enhanced the surface hydrophilicity and solubility of whey protein. Additionally, the incorporation of sugar chain structure introduced steric hindrance, facilitating the binding of the free amino group of lysine and resulting in the formation of macromolecular crosslinking glycation structures. These structures potentially hindered the function of gastrointestinal digestive enzymes, thus reducing the degree of digestion. Furthermore, the gastric and intestinal digests of glycated whey protein variably modulated the intestinal hormone secretion of STC-1 cells and elevated the mRNA levels of pertinent hormones. Derived patterns of the effect of the gastrointestinal digestion products of glycated proteins on the level of hormone secretion in enteroendocrine cells.
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