Study on Purification Process and Antioxidative Activity and α-Glucosidas Inhibitory Activity of Proanthocyanidins from Tetrastigma hemsleyanum
2022
Zhigui HE | Xianglin XU | Yingying CUI | Lan WU | Xiaoyu ZHOU | Zongfang HUANG | Miying DU
In this paper, the purification process and biological activity of proanthocyanidins isolated from Tetrastigma hemsleyanum were explored to prepare economical and efficient proanthocyanidins. The static adsorption and desorption capacity of proanthocyanidins on five kinds of resins were compared. The dynamic adsorption and desorption conditions of resins were optimized. The effects of ethanol elution on the purity and biological activity of proanthocyanidins were evaluated. The results showed that AB-8 resin was chosen as the better sorbent for the purification of proanthocyanidins from Tetrastigma hemsleyanum. The optimal conditions for purifying proanthocyanidins were as follows: Sample concentration 6.00 mg/mL, loading rate 2 BV/h, loading amount 11 BV, eluent flow rate 1.5 BV/h, and eluent volume 2 BV. 70% ethnol had the best effect for the purification of proanthocyanidins, and the purification of proanthocyanidins were 97.31%±0.96%, 2.2 times the purification of crude extract. The DPPH scavenging activity, ABTS+ scavenging activity and α-glucosidase inhibitory activity were also increased dramatically and there was a significant positive correlation between these activities and content of proanthocyanidins（P<0.01）. Therefore, the AB-8 resin purification method was simple and efficient, and the Tetrastigma hemsleyanum proanthocyanidin had strong in vitro antioxidant activity and α-glucosidase inhibitory activity. It would provide a scientific reference for the comprehensive development and utilization of proanthocyanidins in Tetrastigma hemsleyanum.
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