Amplification of Cherimoya (<i>Annona cherimola</i> Mill.) with Chloroplast-Specific Markers: Geographical Implications on Diversity and Dispersion Studies
2022
Nerea Larranaga | Gustavo Fontecha | Federico J. Albertazzi | Margarita Palmieri | Jose I. Hormaza
Previously developed <i>Annona cherimola</i> specific primers based on the plant barcode gene <i>mat</i>K were tested in 546 cherimoya accessions. Of those, 296 belong to an ex situ world reference germplasm collection maintained at the IHSM La Mayora-CSIC-UMA in Málaga (Spain) and 250 to cherimoya samples collected in situ in three Central American countries (Honduras, Guatemala and Costa Rica). Results showed the existence of two different haplotypes in the samples analyzed with geographical association. A new marker that amplified all the samples was designed based on the <i>trn</i>L-F locus using DNA information from seven species of the <i>Annona</i> genus. The information generated will be useful to analyze and conserve the extant genetic diversity present in situ and ex situ and to understand de dispersion of the crop from its center of origin in Central America.
显示更多 [+] 显示较少 [-]